In Yu-Gi-Oh! Duel Monsters 7: The Duelcity Legend and Yu-Gi-Oh! The Sacred Cards, Yami Yugi first appears at the Battle City finals when he defeats Yami Bakura in the quarterfinals. He later faces the player in the semifinals and, if defeated, hands over Slifer the Sky Dragon.
In Yu-Gi-Oh! Duel Monsters 8: Reshef of Destruction and Yu-Gi-Oh! Reshef of Destruction, Yugi's Millennium Puzzle disappears, causing Yami Yugi to disappear too. He appears briefly after retrieving the Puzzle from Bandit Keith, only to disappear again when its smashed to pieces. He appears again after Pegasus gives the pieces back to Yugi and finally once more as a bonus boss in the Hall of Eternity.
In Yu-Gi-Oh! Duel Monsters EX 3 and Yu-Gi-Oh! World Championship Tournament 2004, Yami Yugi is a Level 6 opponent. To unlock him, the player must defeat all Level 1 to 5 opponents at least fifteen times each. After meeting this condition, if the player defeats Yugi three times in a row and challenges him to a fourth Duel, Yami Yugi will Duel instead. If the player defeats Yami Yugi once, he will be unlocked as a regular opponent.
In Yu-Gi-Oh! Duel Monsters International 2 and Yu-Gi-Oh! 7 Trials to Glory: World Championship Tournament 2005, Yami Yugi is unlocked randomly as an opponent after the player beats any Misc. Duelist in Shadow World. He appears at a random location in Battle City or Shadow World once per day. If defeated, he vanishes if you leave the area he’s in or turn off your GBA until you meet his unlock requirements again.