- In episode 100, Breo uses this card during his Turbo Duel against Yusei Fudo. He Synchro Summons this card by tuning "Mine Mole" with "Bicorn Re'em" (who is a Tuner in the anime). Since "Bicorn Re'em" was sent to the Graveyard for a Synchro Summon, its effect activates, forcing Yusei to send the top two cards of his Deck ("Ghost Gardna" and "Reinforce Truth") to the Graveyard. Since "Mine Mole" was sent to the Graveyard as a Synchro Material Monster for the Synchro Summon of a Beast-type Synchro Monster, its effect activates, allowing Breo to draw one card. Yusei and his friends wondered if Breo was going to use the effect of "Speed World 2" to destroy "Stardust Dragon" which would allow Breo to attack Yusei directly this card. Breo, to the surprise of Team 5D's, activates the effect of "Speed World 2" to remove ten Speed Counters and destroy this card. This activates this card's effect, forcing both players to send the top seven cards of their Deck to the Graveyard. It's at this point that Yusei and his friends realize that Breo uses a Mill Deck and that he is planning to defeat Yusei by making him lose via Deck Out. In the next episode, Breo revives this card via "Regretful Tuning". This card then attacks "Stardust Dragon", but Yusei activates "Synchro Striker Unit" to equip it to "Stardust Dragon" and increase its ATK by 1000. Breo then activates "Flattery" to increase this card's ATK by the difference between the original ATK of "Stardust Dragon" and its current ATK. Both battling monsters are then destroyed in a Double KO. The effect of this card then activates, forcing both players to send the top seven cards of their Deck to the Graveyard. Breo then activates "Tuning Collapse" to force Yusei to send eight cards from the top of his Deck to the Graveyard (the number of sent cards is equal to the Level of "Stardust Dragon").
- In episode 102, Jean uses this card during his Turbo Duel against Yusei. He Special Summons this card from his Extra Deck via the effect of "Lightning Tricorn". Later this card attacks "Dragon Knight Draco-Equiste" (Jean intended for this card to be destroyed by "Dragon Knight Draco-Equiste" which would activate this card's effect and force Yusei to send the top seven cards of his Deck to the Graveyard which would make Yusei lose due to Deck Out), but Yusei activates "Scrap-Iron Scarecrow" to negate the attack. Shortly after that, "Scrap-Iron Scarecrow" resets itself face-down. Later "Rapid Warrior" attacks directly via its own effect, but Jean activates "Barrier Wave" to Tribute this card and switch both of Yusei's monsters to Defense Position. After that, the last effect of this card inflicts damage to Yusei equal to the DEF of "Rapid Warrior".
- This card also appears in a flashback Yusei has when he remembers the effect of this card and how it made him send a lot of the cards in his Deck to the Graveyard.
- While Breo is summoning this card, he chants "Galloping in heavens like thunder, tearing apart the sea of clouds! These hooves will rip across the surface of the earth! Synchro Summon! Roar, Voltic Bicorn!"
- In the anime, its effect would activate whenever it was destroyed in general, not just when your opponent destroys it.
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